Abstract Purpose Drawing on affective events theory, this study positions role overload and supervisor frustration as antecedents of abusive supervision, leading to supervisor-led organizational citizenship behavior. In a mediated-moderation-sed analysis, we question whether coworker support moderates the relationship between supervisor frustration and abusive supervision and whether guilt mediates between abusive supervision and supervisor-led OCB. Theoretical framework This research study is inspired by the assumptions of affective events theory. Design/methodology/approach In a time-lagged survey, we collected data from 351 medical supervisors employed in private hospitals in Pakistan. The PROCESS macro is used to perform a mediated-moderation analysis. Furthermore, the research is analyzed based on structural equation modeling (SEM) procedures using the AMOS software, version 26.0. Findings The results demonstrate that role overload predicts supervisor frustration, leading to abusive supervision. Moreover, when coworker support is high, the relationship between supervisor frustration and abusive supervision is weaker. Supervisor frustration and guilt mediate the path from role overload to supervisor frustration, abusive supervision, and supervisor OCB, respectively. Practical & social implications of research Organizational policymakers should consider role overload as an element that causes stress and frustration among medical supervisors, resulting in abusive behavior towards their subordinates. Thus, some normative measures might be appropriate to reduce abusive supervision in the healthcare sector. Socially, this study can help in combating frustration and aggression among working people, representing a significant proportion of Pakistani society. Originality/value The mediators, such as supervisor frustration and guilt, advance our understanding of abusive supervision research.

N-Phthalimido β-amino acid derivatives, 3-phthalimido-3(2-hydroxyphenyl) propanoic acid (P2HPA) and 3-phthalimido-3(2-nitrophenyl) propanoic acid (P2NPA) with new series of di- and triorganotin(IV) complexes (1-12) have been designed and synthesized. All the ligands and organotin(IV) complexes were characterized by elemental analysis, Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (1H, 13C, 119Sn) spectroscopy and electron ionization mass spectrometry (EI-MS). Synthesized ligands and complexes were screened to determine the antibacterial activity and results showed that the triorganotin(IV) complexes have better activity compared to diorganotin(IV) complexes and ligands. In addition, molecular docking analysis of ligands on the catalytic pocket of sortase A (PDB ID 1T2W) showed that the ligands can bind the active amino acid residues in the pocket. The antioxidant activity was also performed by the DPPH (1,1-diphenyl-2-picrylhydrazyl radical) method and complexes showed better results than ligands. The compounds were also tested in vivo to determine the hypoglycemic activities on different groups of alloxan induced diabetic rabbits. The complexes (1-6) were found better hypoglycemic agents as they stabilized the glucose level to about 175-105 mg dL-1 as compared to ligand P2HPA.

Abstract Phthalimide is considered a scaffold for the development of new anticancer agents. In this work, the antiproliferative activity of forty-three phthalimide derivatives was evaluated against cervical (HeLa), liver (HepG2), breast (4T1) cancer cell lines, and a normal cell line of murine fibroblasts (3T3). Finally, a molecular docking analysis of phthalimide derivatives on the active site of the enzymes DNA methyltransferase 1 (DNMT1) and vascular endothelial growth factor receptor 2 (VEGR2) as potential drug targets was performed. The compounds, C16, E11, and E16 showed the best antiproliferative activity against the cell lines HeLa and 4T1. Only, the compound H16 decreased 32% cell proliferation against HepG2 cell line. The compounds H5, H16, E2, E16, and C1 did not affect the proliferation of the 3T3 cell line. The molecular docking analysis showed that phthalimide derivatives have a greater affinity for DNMT1 than S-adenosyl-l-homocysteine, a potent DNMT1 inhibitor. However, molecular docking results do not correlate with their antiproliferative effects, suggesting another potential mechanism of action for the active compounds.

Resumen Laestructura de la ftalimida esconsiderada un bloque de construcción para el desarrollo de nuevos agentes anticancerígenos. En este trabajo, se evaluó la actividad antiproliferativa de cuarenta y tres derivados de ftalimida contra las líneas celulares cancerígenas de cérvix (HeLa), hígado (HepG2), mama (4T1), y la línea celular normal de fibroblastos murinos (3T3). Por último, se realizó un análisis de acoplamiento molecular de los derivados de la ftalimida en el sitio activo de la enzima metiltransferasa 1 de DNA (DNMT1, por sus siglas en inglés) y el receptor del factor de crecimiento endotelial vascular 2 (VEGR2, por sus siglas en inglés) como posibles blancos farmacológicos. Los compuestos C16, E11 y E16 mostraron la mejor actividad antiproliferativa contra las líneas celulares HeLa y 4T1. Solamente, el compuesto H16 disminuyó 32% la proliferación celular de la línea HepG2. Los compuestos H5, H16, E2, E16 y C1 no afectaron la proliferación celular de la línea 3T3. El análisis de acoplamiento molecular demostró que los derivados de la ftalimida tienen una mayor afinidad que la S-adenosil-l-homocisteína, un potente inhibidor de la metiltransferasa 1 de DNA. Sin embargo, los resultados del acoplamiento molecular no se correlacionan con los efectos antiproliferativos; lo cual sugiere que los compuestos activos tienen otro mecanismo de acción.

Resumo Objetivo: Este estudo tem como objetivo mostrar empiricamente os efeitos positivos da liderança ressonante, da amizade no local de trabalho e da cultura de atendimento, prevendo o comprometimento organizacional em um papel mediador da compaixão no trabalho. Metodologia: Os dados foram coletados de 442 funcionários de linha de frente que trabalham em organizações de saúde do Paquistão, empregando uma pesquisa transversal. Os dados coletados foram analisados pela técnica de modelagem de equações estruturais (MEE), usando o SmartPLS 3.0. Resultados: Todas as relações propostas foram estatisticamente fundamentadas. Constatamos que a liderança ressonante, a cultura de atendimento e a amizade no local de trabalho predizem um comprometimento normativo entre os profissionais de saúde em um papel mediador da compaixão no trabalho. Os resultados mostram uma excelente adequação do modelo, no qual todas as hipóteses diretas e indiretas são sustentadas pelos dados. Contribuições: Os efeitos positivos da liderança ressonante, da amizade no local de trabalho e da cultura de atendimento como uma estrutura unificada para prever o comprometimento organizacional por meio do papel mediador da compaixão em um ambiente de trabalho na área da saúde são exclusivos deste estudo.

Abstract Purpose: This study aims to empirically demonstrate the positive effects of resonant leadership, workplace friendship and serving culture on organizational commitment through a mediating role of compassion at work. Design/methodology/approach: The data are collected from 442 front line employees (FLEs) working in Pakistani healthcare organizations by employing a cross-sectional survey. The collected data are analysed through the structural equation modelling (SEM) technique, using SmartPLS 3.0. Findings: All the proposed relationships are statistically supported. We found that resonant leadership, serving culture and workplace friendship predict normative commitment among healthcare frontliners through a mediating role of compassion at work. The results demonstrate an excellent model fit, where all the direct as well as indirect hypotheses are supported by the data. Originality/value: The analysis of the positive effects of resonant leadership, workplace friendship and serving culture as a unified framework to predict organizational commitment via the mediating role of compassion in a healthcare setting is unique to this study.

Abstract Background: Histone acetylation is an important epigenetic modification that regulates gene activity in response to stress. Histone acetylation levels are reversibly regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). The imperative roles of HDACs in gene transcription, transcriptional regulation, growth and responses to stressful environment have been widely investigated in Arabidopsis. However, data regarding HDACs in kenaf crop has not been disclosed yet. Results: In this study, six HDACs genes (HcHDA2, HcHDA6, HcHDA8, HcHDA9, HcHDA19, and HcSRT2) were isolated and characterized. Phylogenetic tree revealed that these HcHDACs shared high degree of sequence homology with those of Gossypium arboreum. Subcellular localization analysis showed that GFP-tagged HcHDA2 and HcHDA8 were predominantly localized in the nucleus, HcHDA6 and HcHDA19 in nucleus and cytosol. The HcHDA9 was found in both nucleus and plasma membranes. Real-time quantitative PCR showed that the six HcHDACs genes were expressed with distinct expression patterns across plant tissues. Furthermore, we determined differential accumulation of HcHDACs transcripts under salt and drought treatments, indicating that these enzymes may participate in the biological process under stress in kenaf. Finally, we showed that the levels of histone H3 and H4 acetylation were modulated by salt and drought stress in kenaf. Conclusions: We have isolated and characterized six HDACs genes from kenaf. These data showed that HDACs are imperative players for growth and development as well abiotic stress responses in kenaf.

Abstract Diospyros kaki L.f. belonging to family Ebenaceae, commonly known as persimmon is used as a medicinal plant in Chinese traditional medicine since many years for different ailments including cosmetics and dermatologic applications. Traditionally this plant is used to treat different skin conditions including pimples, skin eruptions and eczema. Present interest has been focused toward use of natural bioactive compounds in various curative and beautifying applications in dermatological and cosmeceutical disciplines. The objective of this article is to present cumulative data on potential use of D. kaki for its possible role in dermatologic and cosmetic applications. Scientific data has revealed an excellent position of D. kaki in both dermatology and cosmetic discipline making it a valuable choice in respective field. Active principles from different plant parts have shown to possess anti-inflammatory, antiallergic, photo-protective, and anti-wrinkle effects with appreciable activities against tyrosinase, elastase, and collagenase enzymes. Promising antioxidant activity and skin whitening potential, augmented by reduction in sebum contents, and reduction in size and number of skin pores make it a suitable choice as cosmetic ingredient. Data has been summarized and presented on available molecular mechanism that can contribute toward phytoconstituents usage in cosmetics and dermatology mediated by different cellular pathways. Crude extracts and some of phytochemical obtained from this plant such as isoquercitrin and hyperin have better reported activities than well-known cosmetic ingredients viz., arbutin, kojic acid and hydroquinone with possibility of having no side effects. Photo protection against degenerative effects of UVA, UVB and gamma radiation can help skin to fight well against oxidative stress and reactive oxygen species. Further investigation need to be directed toward human subjects for evaluation of these reported activities for obtaining optimum commercial and industrial benefits from this valuable plant.

RESUMOEstudos que medem brand equity de destino turístico utilizando o modelo Customer-Based Brand Equity (CBBE) no contexto de países em desenvolvimento têm sido escassos. O presente artigo investiga a brand equity do Forte Lahore, empregando o modelo CBBE no Paquistão. Seguindo a tradição positivista, realizamos um levantamento com 237 turistas em visita ao Forte Lahore. Os dados foram coletados por meio de um questionário desenvolvido para explicar as relações do conhecimento de marca, imagem de marca, associação de marca, e fidelidade à marca com a brand equity do Forte Lahore. Utilizamos várias técnicas estatísticas robustas, como correlação, regressão e análise fatorial confirmatória (utilizando o método PLS) a fim de chegar a conclusões significativas. Descobrimos que a imagem e as associações da marca contribuem positivamente para a fidelização, por sua vez, contribui significantemente com brand equity. Pragmaticamente, o estudo mede a brand equity com base na percepção de clientes em relação ao Forte Lahore, uma marca de destino turístico. Os resultados são úteis na medida em que sugerem estratégias que podem ajudar responsáveis pelas leis do turismo local a melhorarem o desempenho da marca.

ABSTRACTStudies that measure the brand equity of destination brands by using the Customer-Based Brand Equity (CBBE) model in a developing country context are scarce. The present study investigates the destination brand equity of the Lahore Fort by employing the CBBE model in a developing country context of Pakistan. Following the positivist tradition, we adopted a survey-based approach to collect data from 237 tourists visiting the Lahore Fort. Data were collected through a questionnaire developed to explain the relationship of brand awareness, brand image, brand association, and brand loyalty with Lahore Fort’s overall brand equity. We used various robust statistical techniques such as correlation, regression and confirmatory factor analysis (using PLS method) to reach meaningful conclusions and found that brand image and brand associations positively contribute to brand loyalty. Furthermore, brand loyalty significantly contributes towards overall brand equity. Pragmatically, this study measures the customer based brand equity of the Lahore Fort, a destination brand. The results are useful as they suggest a few strategies that can help policy makers to enhance Lahore Fort’s brand performance.

RESUMENEstudios que miden el valor de marca de marcas de destino utilizando el Customer-Based Brand Equity (CBBE) modelos en el contexto de un país en desarrollo son escasos. El presente estudio investiga la marca de valor de destino del Fuerte Lahore empleando el modelo CBBE en el contexto de un país en desarrollo en Pakistán. Siguiendo la tradición positivista, adoptamos un enfoque basado en encuesta para colectar datos de 237 turistas visitando el Fuerte Lahore. Los datos fueron coletados a través de un cuestionario desarrollado para explicar la relación de conciencia de marca, imagen de marca, asociación de marca, y leadad de marca con el valor de marca total del Fuerte Lahore. Nosotros utilizamos varias técnicas estadísticas robustas como correlación, regresión y análisis de factores confirmativos (utilizando el método PLS) para alcanzar conclusiones significativas y descobrir que el imagen de marca y las asociaciones de marca contribuyen positivamente a la lealtad de marca. Además, la lealtad de marca contribuye significativamente hacia la equidad de marca en general. Pragmáticamente, ese estudio mide el valor de marca basado en el cliente del Fuerte Lahore, una marca de destino. Los resultados son útiles ya que sugieren algunas estrategias que pueden ayudar los responsables por la formación de políticas a aumentar el desempeño de la marca.

Current study investigates the service quality offered by Pakistani news channels through employing a modified SERVQUAL scale. Further to this, the SERVQUAL and its applicability to measure the service quality of news channels has been presented. A 29-item SERVQUAL based instrument was administered to a sample of 318 randomly selected respondents. The descriptive analysis revealed a ‘gap analysis’ model to infer meaningful results. The results reveal several gaps between expected and perceived service quality. In particular, ‘Responsiveness and Assurance’ dimensions had the highest gaps. However, the SERVQUAL scale has been a good fit while measuring the service quality of news channels in a developing country context. This research originally contributes to the existing service quality literature as the SERVQUAL scale has never been used to measure service quality of TV channels. The recommendations will be helpful in minimizing the service quality gaps and also can trigger channel loyalty by offering services in accordance with the viewer expectations.

Several wheat genotypes were screened against heat stress. Seven wheat (Triticum aestivum L.) cultivars obtained after screening against heat classified as tolerant, moderately tolerant, and susceptible to heat stress, were mated in a complete diallel mating system to study the inheritance pattern of relative cell injury percentage (cell injury %) and some yield contributing parameters under normal and heat stress conditions. Significant genotypic differences were found (P < 0.01) for all the studied traits under both regimes. The scaling test disclosed partial adequacy for traits such as days to heading and days to maturity, but for flag leaf area and relative cell injury %, it showed full sufficiency under both environments. The model for grain yield per plant and biomass per plant were fully adequate under normal conditions, but partially adequate under stress. The harvest index showed partial adequacy under normal conditions, but was fully adequate under stress. The additive component of genotypic variation (D) was significant for all studied traits and more significant than the H1 and H2 dominance components. Values of the gene proportion with positive and negative effects in the parents (H2MH1) demonstrated an unequal distribution of dominant genes in the parents for almost all the traits except for flag leaf area, grain yield per plant, and harvest index which showed an equal distribution of dominant genes under stress conditions. High heritability estimates were found for days to heading, days to maturity, flag leaf area, grain yield, and relative cell injury percentage under both regimes. Moderately high estimates were found for biomass per plant and harvest index.

Varios genotipos de trigo se discriminaron para estrés térmico. De ellos siete cultivares de trigo (Triticum aestivum L.) incluyendo tolerantes, moderadamente tolerantes, y susceptibles a estrés térmico obtenidos después de discriminar con calor se aparearon en un sistema de apareamiento de dialelo completo para estudiar el patrón de heredabilidad del porcentaje de daño celular relativo (daño celular %) y algunos parámetros que contribuyen al rendimiento bajo condiciones normales o de estrés térmico. Se encontraron diferencias genotípicas significativas (P < 0.01) para todos los rasgos estudiados bajo ambas condiciones. Pruebas de ajuste revelaron adecuación parcial para rasgos como días a espigadura y días a madurez en ambas condiciones pero mostraron suficiencia completa para área hoja bandera y daño celular % en ambas condiciones. El modelo para producción de grano por planta y biomasa por planta fue completamente suficiente bajo condiciones normales y completamente adecuado en estrés. El componente aditivo de variación genotípica (D) fue significativo para todos los rasgos estudiados y más que los componentes de dominancia H1 y H2. Los valores de la proporción de genes con efectos positivos y negativos en los progenitores (H2/4H1) demostraron distribución desigual de genes dominantes en los progenitores para casi todos los rasgos excepto área de hoja bandera, producción de grano, y daño celular % en ambas condiciones. Se encontraron estimaciones relativamente altas para biomasa por planta e índice de cosecha.